Structure: function analysis of RhoBTB1, an atypical Rho GTPase.

Abstract

RhoBTB1 is an unusual Rho GTPase family member. It binds to the Cullin-3 ubiquitin ligase complex via its BTB domains, and is therefore likely to target proteins for proteasomal degradation. We have recently found that it binds via its Rho domain to components of the Rho signalling pathway involved in cell migration. This project will determine the structure of the Rho domain in complex with Rho pathway components, and of the BTB domains with Cullin-3. The function of RhoBTB1 interactions will be investigated in cells by site-directed mutagenesis, high-resolution microscopy and analysis of protein degradation.





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Biological Areas:

Cell Biology
Structural Biology

BBSRC Area:

Molecules, cells and industrial biotechnology