Primary immune-mediated haemolytic anemia (pIMHA) is a debilitating, potentially fatal, disease in human beings and dogs. Little is known about the cellular events inciting autoaggressive immune responses against red blood cells (RBCs) in either species. Using the dog as model of the human disease, our aim is to test the hypothesis that canine pIMHA is characterized by global deficits of regulatory T cell (Treg) and myeloid-derived suppressor cell (MDSC) function that permit autoaggressive responses of conventional T cells to immunodominant red blood cell (RBC) antigens. We will employ flow cytometry, next generation sequencing by RNA-seq, and in vitro functional assays.
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