Novel quantum dot systems for spectroscopic super-resolution imaging and two-photon excitation microscopy in neuron cells.

Abstract

Deeper understanding of cell physiology and intra- and inter-cellular communications requires significant improvement in both spatial resolution and in image acquisition rate [1, 2]. To this end we propose to develop and test in-vitro novel fluorescent probes based on germanium, gold and carbon quantum dots. Our goal will be to understand and exploit relationships between synthesis conditions, atomic structure and origins of light emission. Probes will be optimised (for size, QY, etc.) and tested in-vitro on neuron cell lines followed by tests in brain slices to assess probe functionality deep in living tissue.





References:
[1]

D.A. Rusakov D.A., Disentangling calcium-driven astrocyte physiology, Nature Rev Neurosci 16: 226-233, (2015)

[2]

R. Scott, C. Henneberger,R. Padmashri,S. Anders,T. Jensen, D.A. Rusakov, Neuronal adaptation involves rapid expansion of the action potential initiation site. Nature Commun 5, 3817, (2014)

[3]

J. Briscoe, A. Marinovic, M. Sevilla, S. Dunn, M. Titirici, Biomass-derived carbon quantum dot sensitizers for solid-state nanostructured solar cells. Angewandte Chemie, 54, 4463-8 (2015)

[4]

A. Karatutlu, M.Y. Song, A. P. Wheeler, O. Ersoy, W.R. Little, Y.P. Zhang, P. Puech, F.S. Boi, Z. Luklinska, A.V. Sapelkin, Synthesis and structure of free-standing germanium quantum dots and their application in live cell imaging, RSC ADVANCES, 5, 20566-20573 (2015)

[5]

M. Song, A. Karatutlu, O. Ersoy, Y. Zhou, Y. Yang, Y. Zhang, W. R. Little, A. P. Wheeler, A. V. Sapelkin, Spectroscopic super-resolution fluorescence cell imaging using ultra-small Ge quantum dots, arXiv:1503.09151v1


Biological Areas:

Neurobiology

BBSRC Area:

Molecules, cells and industrial biotechnology