Gram -ve bacteria shed vesicles from their outer membranes as part of their interactions with eurkarotic hosts. It is clear from previous research that this is an ordered process, with only a subset of proteins being packaged in vesicles. This project aims to understand the molecular basis for the vesicle formation in the dental pathogen Porphyromonas gingivalis and to understand how the selective loading of vesicles is achieved. This project will use molecular microbiology coupled with fluorescent microscopy and electron microscopy to unravel this process and shed light on the biology of the bacterial outer membrane.
M.F. Haurat, Aduse-Opoku J, Rangarajan M, Dorobantu I, Gray MR, Curtis MA, Feldman M (2011) Selective sorting of cargo proteins into bacterial membrane vesicles. J Biol Chem 286,1269-1276.
Chiu SW, Roberts MA, Leake MC, Armitage JP (2013) Positioning of chemosensory proteins and FtsZ through the Rhodobacter sphaeroides cell cycle. Mol Microbiol. 90, 322-37
Nenninger A, Mastroianni G, Robson A, Lenn T, Xue Q, Leake MC, Mullineaux CW (2014) Independent mobility of proteins and lipids in the plasma membrane of Escherichia coli. Mol. Microbiol. 92, 1142-1153.
Liu L-N, Bryan SJ, Huang F, Yu J, Nixon PJ, Rich PR, Mullineaux CW (2012) Control of electron transport routes through redox-regulated redistribution of respiratory complexes. Proc. Natl. Acad. Sci. USA 109, 11431-11436
Losi, A., Gärtner, W., et al. (2013). A photochromic bacterial photoreceptor with potential for super-resolution microscopy. Photochem. Photobiol. Sci. 12, 231–5.