Ribosomes are the primary site of protein translation in the cell and as such are amongst the most important biological molecular machines. The Lto1-Yae1-Rli1 complex is essential the assembly and function of ribosomes and has a role in controlling initiation of translation. One known function of the complex is the mitigation of oxidative damage to the ribosome. This project will structurally and functionally characterize this essential and conserved complex, in order to learn the molecular mechanism of its function in atomic resolution detail and to further understand the ribosomal pathways that it controls and the factors that influence this.
Zhai et al. 2014 The function of ORAOV1/LTO1, a gene that is overexpressed frequently in cancer: essential roles in the function and biogenesis of the ribosome. Oncogene 33(4) p.484-94
Karcher et al. 2008 X-ray Structure of the Complete ABC Enzyme ABCE1 from Pyrococcus abyssi J. Biol. Chem. 283 p.7962-7971
Dever & Green 2012 The elongation, termination, and recycling phases of translation in eukaryotes. Cold Spring Harb Perspect Biol. 2012 Jul 1;4(7):a013706