Infectious diseases remain the leading cause of death worldwide. With bacterial resistance on the rise and the pipeline of new antibiotics depleted, the identification of new antimicrobial targets, especially in Gram-negative pathogens, is of fundamental scientific importance. In this project, we are pursuing a structure-based approach to develop inhibitors for the bacterial glycosyltransferase NGT, a key enzyme for protein N-glycosylation in respiratory pathogens such as Haemophilus influenzae. The new inhibitors will be powerful tools to study the interplay of protein glycosylation and virulence in living bacteria, and an exciting starting point for the development of a new class of “glycobiotics”.
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