Mechanical forces influence many aspects of cell behavior. Mechanotransduction is classically described as the process whereby forces acting on the cell surface are transduced into biochemical functions, eventually reaching the cell nucleus. While much has been studied about the role of focal adhesions and cytoskeletal actin to control cell mechanics, the role of the LINC complex, linking the Nucleoskeleton with the cytoskeleton, has remained elusive. Here we will employ a combination of cell biology, molecular biology and advanced high-resolution Atomic Force Microscopy to dissect the molecular determinants that govern the mechanical stability of individual cells and isolated nuclei.
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