The T cell immunological synapse is a critical structure regulating T cell activation. Here, the formation of microclusters of proximal signalling molecules is known to regulate signalling. We developed sophisticated cluster analysis methods based on single-molecule super-resolution fluorescence microscopy to analyse these structures in detail. This analysis has never been applied to molecules on the other side of the synapse: in the antigen presenting cell (APC). Here too, molecular clustering is thought to play an important role. We therefore propose to investigate how protein clustering in APCs controls signal transduction at the immunological synapse.
Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events. Williamson DJ1, Owen DM, Rossy J, Magenau A, Wehrmann M, Gooding JJ, Gaus K. Nature Immunology 2011
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution Eric Betzig, George H. Patterson, Rachid Sougrat, O. Wolf Lindwasser, Scott Olenych, Juan S. Bonifacino, Michael W. Davidson, Jennifer Lippincott-Schwartz, Harald F. Hess Science 2006
PALM imaging and cluster analysis of protein heterogeneity at the cell surface Dylan M. Owen, Carles Rentero, Jérémie Rossy, Astrid Magenau, David Williamson, Macarena Rodriguez andKatharina Gaus Journal of Biophotonics 2010