Creating Novel Adhesives from genetically programmed directional splicing of recombinantly produced protein modules.

Abstract

We will use intein-mediated protein splicing to assemble specific protein modules from molluscs/bacterial biofilms into novel adhesive fibres and gels. This will be achieved by combining our interdisciplinary research expertise using our exclusive and innovative recombinant fusion protein system and our knowledge on bacterial biofilm and mollusc adhesion proteins. Utilising the recombinant fusion protein system, we will be able to directionally self-assemble protein modules into the desired adhesive. By combining fusions correctly, predictable arrays of adhesive fibres and gels will be formed with controllable properties/architectures. This technology offers a low-cost highly tuneable platform technology to produce novel adhesives.




References:
[1]

Phillips, J. J.; Millership, C.; Main, E. R. Angewandte Chemie 2012, 51, 13132.

[2]

Berry, A. A.; Yang, Y.; Pakharukova, N.; Garnett, J. A.; Lee, W. C.; Cota, E.; Marchant, J.; Roy, S.; Tuittila, M.; Liu, B.; Inman, K. G.; Ruiz-Perez, F.; Mandomando, I.; Nataro, J. P.; Zavialov, A. V.; Matthews, S. PLoS Pathog 2014, 10, e1004404

[3]

Garnett, J. A.; Martinez-Santos, V. I.; Saldana, Z.; Pape, T.; Hawthorne, W.; Chan, J.; Simpson, P. J.; Cota, E.; Puente, J. L.; Giron, J. A.; Matthews, S. Proc Natl Acad Sci U S A 2012, 109, 3950.

[4]

Main, E. R.; Phillips, J. J.; Millership, C. Biochem Soc Trans 2013, 41, 1152.

[5]

Gowers SAN, Curto VF, Seneci CA, Wang C, Anastasova S, Vadgama P, Yang G-Z and Boutelle MG (2015). 3D Printed Microfluidic Device with Integrated Biosensors for Online Analysis of Subcutaneous Human Microdialysate.Analytical Chemistry  vol. 87, (15) 7763-7770


Biological Areas:

Chemical Biology
Biotechnology

BBSRC Area:

Genes, development and STEM approaches to biology
Molecules, cells and industrial biotechnology