We found that transcription factors Egr2 and 3 have reciprocal functions in regulating proliferation and differentiation of T cells. Egr2 and 3 deficiency in T cells leads to severe infection and also the development of auto-immune diseases. Based on our knockout and knockin models, this project aims to analyse the data established from CHIP-seq, RNA-seq, and T cell repertoire seq of Egr2 positive T cells to define the target genes of Egr2, the pathways mediated by Egr2 in T cells under different responsive conditions and to create models of the regulatory program in T cell subsets with distinct functions.
Suling Li, Tizong Miao, Meera Sebastian, Punamdip Bhullar, Emma Ghaffari, Mengya Liu, Alistair L. J. Symonds, and Ping Wang, Egr-2 and -3 are essential for both the control of inflammatory autoimmune diseases and antigen receptor mediated activation of B and T cells, Immunity,19;37(4):685-96, 2012.
Miao T, Raymond M, Bhullar P, Ghaffari E, Symonds AL, Meier UC, Giovannoni G, Li S, Wang P Early growth response gene-2 controls IL-17 expression and Th17 differentiation by negatively regulating Batf. J Immunol. 2013;190:58-65.
Ogbe A, Miao T, Symonds AL, Omodho B, Singh R, Bhullar P, Li S, Wang P. Early Growth Response Genes 2 and 3 Regulate the Expression of Bcl6 and Differentiation of T Follicular Helper Cells. J Biol Chem. 2015;290:20455-65.
Kittas A, Barozet A, Sereshti J, Grabe N, Tsoka S, “CytoASP: a Cytoscape App for Qualitative Consistency Reasoning, Prediction and Repair in Biological Networks”, BMC Systems Biology, 9, 34, 2015.
Bennett L, Kittas A, Muirhead G, Papageorgiou LG, Tsoka S, “Detection of Composite Communities in Multiplex Biological Networks”, Scientific Reports, 5:10345, 2015