What happens when the circadian clock ticks in cyanobacteria?


This project will encompass cell biology, molecular biology, and biophysics, including spectroscopy and molecular microscopy to investigate the functional consequences of temporal cycling due to the circadian clock in cyanobacterium Synechococcus elongatus PCC 7942.  This will involve interdisciplinary studies for designing and characterizing "clock protein" knock-out mutants, then using state-of-the-art physical methods (including measurements at high end international research facilities) to characterize these cyanobacteria, altering ambient conditions to establish the effect that the surrounding medium has on the circadian clock.  Additionally the project will trace the extent of linkage between the clock and the functions of secretion and motility.


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Wallace, B.A. and Janes, R.W. Synchrotron radiation circular dichroism (SRCD) spectroscopy: an enhanced method for examining protein conformations and protein interactions (2010) Biochem. Soc. Trans. 38:861-873.


Wilde, A. and Mullineaux, C.W. Motility in cyanobacteria: polysaccharide tracks and Type IV pilus motors (2015) Mol. Microbiol. 98:998-1001.


Mullineaux, C.W. and Nuernberg, D.J. Tracing the path of a prokaryotic paracrine signal (2014) Mol. Microbiol.  94:1208-1212.


Liu, L-N. ……. Mullineaux, C.W. Control of electron transport routes through redox-regulated redistribution of respiratory complexes (2012) Proc. Nat. Acad. Sci. 109:11431-11436.

Biological Areas:

Cell Biology
Structural Biology


Molecules, cells and industrial biotechnology