We will develop novel image analysis algorithms to lineage trace neural stem cells and their progeny in the adult brain assessed as an intact biological system. Tissue clearing methods will be applied to the Confetti model upon activation in neural stem cells (GLASTCreER line) in the adult mouse brain, and Definiens-based analysis of time series of 3D images will be used to assess self-renewal, differentiation and migration in vivo.
Tomer et al. Nature Protocols 2014
Jaunmuktane et al. Nature 2015
Snippert et al. Science 2010
Cai et al. Nature Methods 2013