Advanced image analysis for 3D single cell lineage tracing.

Abstract

We will develop novel image analysis algorithms to lineage trace neural stem cells and their progeny in the adult brain assessed as an intact biological system. Tissue clearing methods will be applied to the Confetti model upon activation in neural stem cells (GLASTCreER line) in the adult mouse brain, and Definiens-based analysis of time series of 3D images will be used to assess self-renewal, differentiation and migration in vivo.




References:
[1]

Tomer et al. Nature Protocols 2014

[2]

Jaunmuktane et al. Nature 2015

[3]

Snippert et al. Science 2010

[4]

Cai et al. Nature Methods 2013


Biological Areas:

Neurobiology
Ageing

BBSRC Area:

Genes, development and STEM approaches to biology