Identification of vaccine candidates in Mycobacterium bovis

Abstract

Mycobacterium bovis causes tuberculosis (TB) in domestic cattle and, in the UK, control of bovine TB is estimated to cost £100M annually. Currently there is no effective vaccine and those based on Mycobacterium bovis BCG, the vaccine licensed for use against human TB, shows limited efficacy. This project will identify and verify genes essential for survival of M. bovis in vivo thereby providing novel vaccine candidates with the potential to contribute to more cost-effective control programs.   




References:
[1]

van Opijnen T, Camilli A. Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms. Nat Rev Microbiol. 2013 Jul;11(7):435-42. doi: 10.1038/nrmicro3033. Epub 2013 May 28. PubMed PMID: 23712350; PubMed Central PMCID: PMC3842022.  

[2]

Hewinson RG, Vordermeier HM, Smith NH, Gordon SV. Recent advances in our knowledge of Mycobacterium bovis: a feeling for the organism. Vet Microbiol. 2006 Feb 25;112(2-4):127-39. Epub 2005 Dec 27. Review. PubMed PMID: 16384663.

[3]

Moule MG, Hemsley CM, Seet Q, Guerra-Assunção JA, Lim J, Sarkar-Tyson M, Clark TG, Tan PB, Titball RW, Cuccui J, Wren BW.Genome-wide saturation mutagenesis of Burkholderia pseudomallei K96243 predicts essential genes and novel targets for antimicrobial development. MBio. 2014 Feb 11;5(1):e00926-13. doi: 10.1128/mBio.00926-13. PubMed PMID: 24520057; PubMed Central PMCID: PMC3950516


Biological Areas:

Microbiology
Genetics

BBSRC Area:

Animal disease, health and welfare