Horror Autotoxicus: The Role of Regulatory T Cells and Myeloid-Derived Suppressor Cells in a Canine Model of Primary Immune-Mediated Haemolytic Anemia


Primary immune-mediated haemolytic anemia (pIMHA) is a debilitating, potentially fatal, disease in human beings and dogs. Little is known about the cellular events inciting autoaggressive immune responses against red blood cells (RBCs) in either species. Using the dog as model of the human disease, our aim is to test the hypothesis that canine pIMHA is characterized by global deficits of regulatory T cell (Treg) and myeloid-derived suppressor cell (MDSC) function that permit autoaggressive responses of conventional T cells to immunodominant red blood cell (RBC) antigens. We will employ flow cytometry, next generation sequencing by RNA-seq, and in vitro functional assays. 


Swann JW, Skelly BJ. Systematic review of prognostic factors for mortality in dogs with immune-mediated hemolytic anemia. J Vet Intern Med 2015, 29(1): 7-13.


Corato A, Shen CR, Mazza G, Barker RN, Day MJ. Proliferative responses of peripheral blood mononuclear cells from normal dogs and dogs with autoimmune haemolytic anaemia to red blood cell antigens. Vet Immunol Immunopathol 1997, 59(3-4): 191-204


Mqadmi A, Zheng X, Yazdanbakhsh K. CD4+CD25+ regulatory T cells control induction of autoimmune hemolytic anemia. Blood 2005, 105(9): 3746-3748.


Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, Chadbourne AH, Palmer CA, Armitage-Chan E, Thompson I, Williamson L, Cunningham F, Garden OA. Phenotypic and functional characterization of a CD4+ CD25high FOXP3high regulatory T-cell population in the dog. Immunology 2011, 132(1): 111-122


Garden OA, Pinheiro D, Cunningham F. All creatures great and small: regulatory T cells in mice, humans, dogs and other domestic animal species. Int Immunopharmacol 2011, 11(5): 576-588

Biological Areas:

Cell Biology


Animal disease, health and welfare