The development of improved antibacterial strategies is an important global challenge. This project aims to develop an effective new scaffold for topical antimicrobial and immunomodulatory therapy. This aim will be achieved by developing electrospun nanofibous polymer membranes that provide 1) flexible scaffolds, 2) inherent antimicrobial properties, and 3) controlled release of incorporated TLR-4 antagonists. The fibres and membranes will be characterized using imaging methods. The antibacterial and immune modulatory effects will be tested in vitro and in vivo, using a murine model of diabetic +/- ischaemic wound healing.
W. Song, G. Mitchell, K. Burugapalli, Electrospinning for Biomedical Applications, in Electrospinning: Principles, Practics and Possibilities, Ed: Geoffrey Mitchell, Royal Society of Chemistry, 2014
Kamaruzzaman, Nor Fadhilah; Firdessa, Rebuma; Good, Liam. (2016) Bactericidal effects of polyhexamethylene biguanide against intracellular Staphylococcus aureus EMRSA-15 and USA 300. Journal of Antimicrobial Chemotherapy. 71(5):1252-1259, May 2016.
Chindera, K., Mahato, M., Sharma, AK., Horsley, H., Kloc-Muniak, K., McFarlane, A., Stach, J., Bentin, T., Good, L. (2016). The antimicrobial polymer PHMB enters cells and selectively condenses bacterial chromosomes. Nature Scientific Reports, 6, 23121. Retrieved from http://dx.doi.org/10.1038/srep23121
Portou, M. J., Yu, R., Shi-wen, X., Abraham, D., Baker, D., & Tsui, J. (2015). Diabetes mellitus exaggerates ischaemia induced inflammation and subsequent tissue damage. BRITISH JOURNAL OF SURGERY, 102, 17.
Navi, A., Abraham, D., Shi-Wen, X., Shaw, S., Baker, D., & Tsui, J. (2014). Role of toll-like receptor 4 (TLR4) inhibition in ischaemia-induced systemic inflammatory response and skeletal muscle damage. BRITISH JOURNAL OF SURGERY, 101, 31.