A game of colonies: how is the balance of proliferation vs differentiation regulated in the human stem like cells?


Stem cell self-renewal and differentiation regulate tissue homeostasis. We will develop a mathematical model to elucidate how key regulators of stem cell self-renewal, namely Wnt and Hippo signaling networks, influence cell commitment for differentiation. We have high-resolution and high-throughput datasets describing functional (intracellular calcium release, [Ca2+]i) and genomic (ChIP-seq, RNA-seq) characteristics in human cells. The motivation for this project is to integrate the genomic and functional biological parameters in a lattice based model. This will allow us to predict 1. how perturbations to these signaling networks interfere with the tissue structure and 2. how that can lead to disease.


Schluter DK, Ramis-Conde I, Chaplain MAJ. 2015 Multi-scale modelling of the dynamics of cell colonies: insights into cell-adhesion forces and cancer invasion from in silico simulations. J. R. Soc. Interface (2014)


Thrasivoulou, C et al, Activation of Intracellular Calcium by Multiple Wnt Ligands and Translocation of β-Catenin into the Nucleus J. Biol. Chem (2013)


Jones, P.H. & Watt, F.M. Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73, 713-724 (1993).


Meng Z, Moroishi T, Guan KL. Mechanisms of Hippo pathway regulation. (2016)


Beaver CM, Ahmed A, Masters JR (2014) Clonogenicity: Holoclones and Meroclones Contain Stem Cells. PLoS ONE 9(2): e89834. doi: 10.1371/journal.pone.0089834

Biological Areas:

Cell Biology


Genes, development and STEM approaches to biology