The biophysics of cell motility in vivo.

Abstract

A better understanding of the mechanisms behind cell motility is crucial to controlling pathologies (e.g. cancer) and for understanding embryonic development. However, most of our knowledge of cell migration stems from in vitro motility assays of limited physiological relevance. In this project we will exploit the embryonic migration of Drosophila macrophages as an in vivo motility system as these cells can be imaged live during development at an unprecedented resolution. We will combine the genetic tractability of this system, computer vision techniques, and computational models to examine the biophysics of migrating cells within this in vivo context.




References:
[1]

Davis J, Luchici A, Mosis F, Thackery J, Salazar J, Mao M, Dunn G, Betz T, Miodownik M, Stramer B. (2015) Inter-cellular Forces Orchestrate Contact Inhibition of Locomotion.  Cell. 161:361-373.

[2]

Davis J, Huang C, Zanet J, Harrison S, Rosten E, Cox S, Soong D, Dunn G, Stramer B.  (2012)  Emergence of embryonic pattern through contact inhibition of locomotion.  Development. 139: 4555-4560.

[3]

Stramer B, Moreira S, Millard T, Evans I, Sabet O, Milner M, Martin P, Wood W.  (2010)  Clasp mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo.  J. Cell Biol. 189:681-689  

[4]

Stramer B, Wood W, Galko M, Redd M, Jacinto A, Parkhurst S, and Martin P. (2005) Live imaging  of wound inflammation in Drosophila  embryos reveals key roles for small GTPases during in vivo cell migration. J. Cell. Biol. 168:567-573.

[5]

Zanet J, Jayo A, Plaza S, Millard T, Parsons M, Stramer B. (2012)  Fascin promotes filopodia formation independent of its role in actin-bundling.  J. Cell Biol. 197:477-486


Biological Areas:

Cell Biology
Development

BBSRC Area:

Molecules, cells and industrial biotechnology