A sustainable and environmentally friendly approach using transketolase biocatalysts for the manufacturing of ketone privileged scaffolds will be developed. Transketolases (TK) represent an attractive class of enzymes which have found applications in biological chemistry due to their ability to catalyse the condensation of hydroxypyruvate with aldehydes. This project will expand their potential by developing new biocatalysts through the combination of chemical biology and synthetic biology approaches. The new enzymes will lead to (di)-carbonyl compounds via Stetter-like 1,4-condensations in a sustainable and environment friendly way.
H. C. Hailes, D. Rother, M. Müller, R. Westphal, J. M. Ward, J. Pleiss, C. Vogel and M. Pohl, FEBS J., 2013, 280, 6374–6394
F. Subrizi, M.Cárdenas-Fernández,. G. J. Lye,. J. M. Ward,. P. A. Dalby, T. D. Sheppard, H. C. Hailes, Green Chem., 2016, 18, 3158-3165
C. Dresen, M. Richter, M. Pohl, S. Lüdeke, M. Müller, Angew. Chem. Int. Ed. 2010, 49, 6600–6603.
S. Bhakta, N. Scalacci, A. Maitra, A.K. Brown, S. Dasugari, D. Evangelopoulos, T. McHugh, P.N. Mortazavi, A. Twist, E. Petricci, F. Manetti, D. Castagnolo, J. Med. Chem. 2016, 59, 2780–2793.
E. Fullam, F. Pojer, T. Bergfors, T. A. Jones, S. T. Cole, Open Biol. 2011, 2, 110026.