An integrative approach to evaluate physiological health by characterizing microbiome and host interaction using saliva samples.

Abstract

The microbiome is involved in human metabolism, nutrition, physiology, and immune function therefore associated with both health and disease1. Among human microbiome populations, saliva’s microbiome provides a powerful non-invasive option for developing biomarkers describing health and disease. Our aim is to use novel statistical integrative approaches while combining host (mRNA) and microbiome (mRNA and DNA) saliva-derived profiles to identify novel non-invasive biomarkers. In this project we will functionally characterize the salivary microbiome in healthy individuals based on age and diet; secondly, we will challenge the ability of saliva’s microbiome to describe health by comparing to individuals with chronic intestinal disease.




References:
[1]

Qin, N., Yang, F., Li, A., Prifti, E., Chen, Y., Shao, L., … Li, L. (2014). Alterations of the human gut microbiome in liver cirrhosis. Nature, 513(7516), 59–64. doi:10.1038/nature13568

[2]

Combs, P. A., & Eisen, M. B. (2015). Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols. PeerJ, 3, e869. http://doi.org/10.7717/peerj.869

[3]

Pandit, P., Cooper-White, J., & Punyadeera, C. (2013). High-Yield RNA-Extraction Method for Saliva. Clinical Chemistry, 20(I), 113–120. doi:10.1089/tmj.2010.0059

[4]

Nielsen, H. B., Almeida, M., Juncker, A. S., Rasmussen, S., Li, J., Sunagawa, S., … Ehrlich, S. D. (2014). Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes. Nature Biotechnology, 32(8), 822–828. doi:10.1038/nbt.2939

[5]

Franzosa, E. a., Hsu, T., Sirota-Madi, A., Shafquat, A., Abu-Ali, G., Morgan, X. C., & Huttenhower, C. (2015). Sequencing and beyond: integrating molecular “omics” for microbial community profiling. Nature Reviews. Microbiology, 13(6), 360–72. http://doi.org/10.1038/nrmicro3451


Biological Areas:

Microbiology
Ageing

BBSRC Area:

Plants, microbes, food and sustainability