Modeling muscle function with bioengineered myofibres derived from pluripotent stem cells

Abstract

Muscles execute all movements and are the main drivers of animal behaviour. While muscle development, physiology and regeneration are well understood, the recreation of muscle from its constituent cellular and extracellular components is still in its infancy. We propose a project which will explore new ways to model muscle function with constructs assembled from pluripotent stem cell-derived muscle cells and artificial scaffolds. To control contractions, we will emulate synaptic input with optogenetic. The aim of the project is to develop 3D-muscle constructs and then apply the technology to study muscle degeneration and explore bioengineered muscle as bio-actuators in soft robots.




References:
[1]

Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci. 2005. 8(9):1263-1268.

[2]

Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, Burrone J, Greensmith L, Lieberam I. Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice. Science. 2014. 344(6179):94-97.

[3]

Chal J, Oginuma M, Al Tanoury Z, Gobert B, Sumara O, Hick A, Bousson F, Zidouni Y, Mursch C, Moncuquet P, Tassy O, Vincent S, Miyanari A, Bera A, Garnier JM, Guevara G, Hestin M, Kennedy L, Hayashi S, Drayton B, Cherrier T, Gayraud-Morel B, Gussoni E, Relaix F, Tajbakhsh S, Pourquié O. Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. Nat Biotechnol. 2015. 33:962-969

[4]

N. Wang, K. Burugapalli, W. Song, J. Hall, F. Moussy, A. Ray, Y. Zheng, Electrospun Polyurethane Nano-fibrous Coating for Glucose Biosensor, Biomaterials, 2013, 34(4):888-901.

[5]

X. Zhang and W. Song, P. Harris, G. Mitchell, T. Bui, and   A. Drake, Chiral Polymer Carbon Nanotube Composite Nanofibres, Advanced Materials, 2007, 19, 1079


Biological Areas:

Cell Biology
Development

BBSRC Area:

Genes, development and STEM approaches to biology