Experimental and computational analysis of tissue elongation

Abstract

Using stem cells to regenerate tissues requires that we know how tissues are built up in normal development. We have previously shown that tissue growth – specifically elongation – involves not just proliferation but also cell shape change and rearrangement. To understand these processes better, this project is to analyse the self-organisation of cell aggregates both in vivo and in silico. The Green lab’s embryonic cell self-elongating aggregate method will be used to test the engineering parameters of elongation compared with those of a computer model developed by the Miodownik group to test our assumptions and improve our understanding of tissue-shaping processes in vivo




References:
[1]

Green, JBA, Davidson, L.,Dominguez, I. (2004) Self-organization of vertebrate mesoderm based on simple boundary conditions. Developmental Dynamics. 231(3): 576-81. http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20163/abstract

[2]

Ninomiya H, Elinson RP, Winklbauer R.(2004) Antero-posterior tissue polarity links mesoderm convergent extension to axial patterning. Nature 430(6997):364-7

[3]

Ninomiya H., Winklbauer R. (2008). Epithelial coating controls mesenchymal shape change through tissue-positioning effects and reduction of surface-minimizing tension. Nat. Cell Biol. 10, 61-69

[4]

Hopyan S, Sharpe J, Yang Y. (2011) Budding behaviors: Growth of the limb as a model of morphogenesis. Developmental Dynamics. 240(5):1054-62.


Biological Areas:

Cell Biology
Development

BBSRC Area:

Genes, development and STEM approaches to biology