Using stem cells to regenerate tissues requires that we know how tissues are built up in normal development. We have previously shown that tissue growth – specifically elongation – involves not just proliferation but also cell shape change and rearrangement. To understand these processes better, this project is to analyse the self-organisation of cell aggregates both in vivo and in silico. The Green lab’s embryonic cell self-elongating aggregate method will be used to test the engineering parameters of elongation compared with those of a computer model developed by the Miodownik group to test our assumptions and improve our understanding of tissue-shaping processes in vivo
Green, JBA, Davidson, L.,Dominguez, I. (2004) Self-organization of vertebrate mesoderm based on simple boundary conditions. Developmental Dynamics. 231(3): 576-81. http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20163/abstract
Ninomiya H, Elinson RP, Winklbauer R.(2004) Antero-posterior tissue polarity links mesoderm convergent extension to axial patterning. Nature 430(6997):364-7
Ninomiya H., Winklbauer R. (2008). Epithelial coating controls mesenchymal shape change through tissue-positioning effects and reduction of surface-minimizing tension. Nat. Cell Biol. 10, 61-69
Hopyan S, Sharpe J, Yang Y. (2011) Budding behaviors: Growth of the limb as a model of morphogenesis. Developmental Dynamics. 240(5):1054-62.