Diffusional analysis of synaptic vesicles: Elucidating the active transport of single vesicles and its relation with their membrane topology

Abstract

Synaptic vesicles (SV) are 40-50 nm membrane organelles that transport and release neurotransmitters by fusing with the synaptic membrane. Their dynamics is critical to control synaptic activity and is linked to molecular motors. However, SV speed is 2-5 fold higher than a typical motor-driven movement. Herein we hypothesize that their diffusion is also controlled by the specific distribution of membrane proteins on their surface creating localized propulsion regimes. The project will involve the purification and characterization of SV by light scattering and electron microscopy, as well as the exploitation of diffusional analysis tracking packages to evaluate any anomalous diffusion pattern

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Biological Areas:

Neurobiology
Biotechnology

BBSRC Area:

Genes, development and STEM approaches to biology
Molecules, cells and industrial biotechnology