Identification of intracellular NHE binding partners and analysis of their cellular function

Abstract

Late endosome/ multivesicular body protein sorting is essential for the correct degradation of membrane proteins during metabolism and cell signalling. Previously, we have shown that the sodium/ proton exchanger NHE8 is involved in protein sorting at multivesicular bodies. This project will investigate the precise role of NHE8 in endosomal protein sorting by studying known and yet to be identified binding partners of NHE8. This project will allow the student to become proficient in cell biological and biochemical techniques (including imaging of live and fixed cells), as well as learning techniques of mass spectrometry.

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References:
[1]

Lawrence S P, Bright N A, Luzio J P and Bowers K. 2010. The sodium/ proton exchanger NHE8 regulates late endosomal morphology and function. Molecular Biology of the Cell, 21: 3540-3551.

[2]

Bowers K, Levi B P, Patel F I, and Stevens T H. 2000. The sodium/ proton exchanger Nhx1p is required for endosomal protein trafficking in the yeast Saccharomyces cerevisiae. Molecular Biology of the Cell, 11: 4277-4294.

[3]

Patel, V. J.*, Thalassinos, K.*, Slade, S. E., Connolly, J. B., Crombie, A., Murrell, J. C. and Scrivens, J. H. (2009). A comparative, qualitative and quantitative proteomic study of the methanotrophic bacterium Methylocella silvestris. Journal of Proteome Research 8: 3752–3759. * joint first authors.

[4]

Thalassinos, K., Vissers, J. P. C., Tenzer, S., Levin, Y., Thompson, J. W., Daniel, D., et al. (2012). Design and application of a data-independent precursor and product ion repository. Journal of the American Society for Mass Spectrometry, 23, 1808–1820.


Biological Areas:

Cell Biology
Structural Biology

BBSRC Area:

Molecules, cells and industrial biotechnology