Hypoglycin A, its metabolites and their binding proteins in Equine Atypical Myopathy

Abstract

Atypical Myopathy (AM) is an acquired fatal disease of horses. Very little is known about the disease but recent research has concluded that AM is caused by hypoglycin A. We will quantify hypoglycin A and metabolites in seeds and leaves during different seasons and locations using triple quadrupole mass spectrometer. The metabolic pathways of hypoglycin A and the proteins it binds to are yet to be discovered. We propose to use previously developed affinity-enrichment proteomics to isolate specific binding proteins. Additionally we will use the precursor ion scale mode to identify potential metabolites of  hypoglycin in serum.

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Biological Areas:

Cell Biology
Chemical Biology

BBSRC Area:

Animal disease, health and welfare