Atypical Myopathy (AM) is an acquired fatal disease of horses. Very little is known about the disease but recent research has concluded that AM is caused by hypoglycin A. We will quantify hypoglycin A and metabolites in seeds and leaves during different seasons and locations using triple quadrupole mass spectrometer. The metabolic pathways of hypoglycin A and the proteins it binds to are yet to be discovered. We propose to use previously developed affinity-enrichment proteomics to isolate specific binding proteins. Additionally we will use the precursor ion scale mode to identify potential metabolites of hypoglycin in serum.
D.-M. Votion, ISRN Vet. Sci., 2012, 2012, 281018.
G. van Galen, C. Saegerman, C. Marcillaud Pitel, F. Patarin, H. Amory, J. D. Baily, D. Cassart, V. Gerber, C. Hahn, P. Harris, J. A. Keen, N. Kirschvink, L. Lefere, B. McGorum, J. M. Muller, M. T. Picavet, R. J. Piercy, K. Roscher, D. Serteyn, L. Unger, J. H. van der Kolk, G. van Loon, D
I. N. Cruz, C. S. Barry, H. B. Kramer, C. Chuang, S. Lloyd, A. van den Spoel, F. M. Platt, M. Yang and B. G. Davis, Chem. Sci., 2013, 4, 3442-3446