Cell and tissue targeting of siRNA using non-viral, biodegradable nanoparticles

Abstract

Controlled and efficient cell and tissue delivery remains an enormous technical challenge, limiting many biotechnology and health applications. This project aims to increase the local delivery and cellular uptake of siRNA molecules. This will be achieved by incorporation within biodegradable polyester/polyhexanide nanoparticles. The siRNA will target tyrosine kinase, providing a novel and specific biological for inhibition of VEGF. Three work packages include: 1) siRNA loading nanoparticles and stability studies, 2) cell delivery, efficacy and mechanism of action studies in liver and vascular tumour cell lines 3) Tissue delivery, efficacy and mechanism of action studies in an ex vivo tissue perfusion model.

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Biological Areas:

Cell Biology
Biotechnology

BBSRC Area:

Genes, development and STEM approaches to biology
Molecules, cells and industrial biotechnology