Viruses are elegant macromolecular assemblies that rely on a limited number of components to complete their replicative cycle and build an infectious particle. Amongst mammalian viruses, Poxviruses are the largest and most complex. They encode for more than 200 genes with 100 different viral proteins contributing to the virion structure. This project aims to unravel this complexity by combining the development of virus targeting nanobodies with experimental superresolution microscopy. We will map the poxvirus nano-architecture by bridging near-molecular scale imaging with a robust mathematical-modeling framework. These studies will advance superresolution technologies while increasing our understanding of virus architecture and infection.
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