Zebrafish offer several key advantages for genetics studies, since they are amenable to high-end genetic manipulation using N-ethyl-N-nitrosourea (ENU) mutagenesis, rapid targeted gene 'knockdown' using antisense morpholinos and most recently, somatic mutagenesis via CRISPR (clustered regularly interspaced short palindromic repeats) technology. In this project, we will create mutants of key members of the SLC6 family of Na+/Cl--dependent symporters (co-transporters) that use the transmembrane electrochemical gradient to actively import neurotransmitters and other substrates into cells. Generating this mutant resource will accelerate our ability to understand the roles of these proteins and their involvement in complex biological processes.
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