Investigating the dynamics of kinase activity and transcription in stem cells using live cell imaging

Abstract

The activity of individual genes and signaling components is highly variable between cells. This variability underlies differing responsiveness of equivalent cells to extracellular triggers in development and disease. It is unclear how this variability evolves. Are distinct signaling and gene expression states defined by long timescales? Or do the activities fluctuate rapidly, and if so, how do fluctuations of signals and their downstream target genes relate to each other? To address these questions, we will used advanced microscopy techniques on living cells to monitor the activity of specific signaling proteins combined with direct measurements of transcriptional dynamics of single target genes.

ject details are protected. Please login with a valid account to view them.





References:
[1]

1.Molecular Cell 52: 529-40

[2]

2.PNAS 109:7350-5

[3]

3.Cell 152:945


Biological Areas:

Cell Biology
Development

BBSRC Area:

Genes, development and STEM approaches to biology