In the last few years, the field of nanobiotechnology has been revolutionised by the emergence of a variety of novel nanomaterials such as graphene, the single atom thick carbon sheet, which attracted attention in the area of biomedicine. A number of staining-free non-destructive microscopy techniques have become available to non-invasively image graphene nanocarriers in biological samples with sub-cellular resolution. The main goal of this research proposal is to develop a dual imaging platform based on Raman spectroscopy and multiphoton bioluminescence to gain understanding on interactions of nanographene with phagocytic cells which constitute the first defense line of our immune system.
C.K.Chua and M.Pumera. Covalent chemistry on graphene. Chemical Society Reviews. 42:3222-3233 (2013).
J.Q.Liu, L.Cui, and D.Losic. Graphene and graphene oxide as new nanocarriers for drug delivery applications. Acta Biomaterialia. 9:9243-9257 (2013).
B.Littleton, T.Kavanagh, F.Festy, and D.Richards. Spectral Interferometric Implementation with Passive Polarization Optics of Coherent Anti-Stokes Raman Scattering. Physical Review Letters. 111 (2013).
A.Almahdy, F.C.Downey, S.Sauro, R.J.Cook, M.Sherriff, D.Richards, T.F.Watson, A.Banerjee, and F.Festy. Microbiochemical Analysis of Carious Dentine Using Raman and Fluorescence Spectroscopy. Caries Research. 46:432-440 (2012).
G.O.Fruhwirth, S.Ameer-Beg, R.Cook, T.Watson, T.Ng, and F.Festy. Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells. Optics Express. 18:11148-11158 (2010).