IgE is the least abundant class of antibodies in the circulation and its normal physiology and homoeostasis are poorly understood despite decades of work focusing on its pathophysiological role in allergy.
Success in discovering these mechanisms will depend on a solid foundation of robust, carefully engineered and well-characterized tool proteins, the critical prerequisite for subsequent in vivo experiments. Rational approaches to engineering the receptor binding sites on IgE based on our crystal structures of IgE with its receptors will be combined with screening novel mammalian cell display libraries and deep sequencing of the protein repertoires.
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