Structural and Functional investigations of LARP6, and its role in degenerative muscle disorders

Abstract

LARP6 is a recently discovered RNA-binding protein involved in regulation of collagen production and muscle differentiation, although little is known about its molecular mechanism(s) of action or its in vivo RNA targets.
The aim of this project is to investigate how LARP6 regulates gene expression at post- transcriptional level during muscle development by using a combination of biophysical, cellular and genetic techniques.

Since aberrant muscle development and collagen production could be linked to neuromuscular disorders and collagen-related diseases, this study will contribute to elucidate any role of LARP6 in degenerative muscle pathologies. 

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References:
[1]

Bayfield, M.A. et al., Conserved and divergent features of the structure and function of La and La- related proteins (LARPs). Biochim Biophys Acta, 2010. 1799:365-78.

[2]

Kotik-Kogan, O., et al., Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein. Structure, 2008. 16: 852-62. 

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Fritz, D., et al., Progress Towards Discovery of Antifibrotic Drugs Targeting Synthesis of Type I Collagen. Curr Med Chem, 2011. 18:3410-6.

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Sugimoto, Y. et al. (2012) Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions. Genome Biol., 2012, 13:R67.

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Sander, J.D. et al. Selection-free zinc-fingernuclease engineering by context-dependent assembly (CoDA). Nature Methods, 2011, 8(1):67-9. 


Biological Areas:

Structural Biology

BBSRC Area:

Genes, development and STEM approaches to biology
Molecules, cells and industrial biotechnology