Mechanical forces influence many aspects of cell behaviour. Mechanotransduction is classically described as the process whereby forces acting on the cell surface are transduced into biochemical functions, eventually reaching the cell nucleus. While much has been studied about the role of focal adhesions and cytoskeletal actin to control cell mechanics, the roles of other main players such as lipid membranes or the LINC complex have remained elusive. Here we will employ a combination of cell biology, molecular biology and advanced high-resolution biophysical techniques to dissect the molecular determinants that govern the mechanical stability of individual cells and isolated nuclei.
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