The protein alpha1-antitrypsin has important anti-inflammatory roles. Some of these relate to its best-understood function (protease inhibition), but others do not. Thus we have recently characterized a central role for alpha1-antitrypsin in immune cell responses to VitaminD. Our data support the importance of a direct interaction between alpha1-antitrypsin and a complement component. This project will characterise the interaction by mass-spectrometry, NMR and crystallographic studies of ex vivo and recombinant samples. We will also search for further alpha1-antitrypsin:cytokine interactions of pathophysiological relevance MS proteomics of patient samples and healthy controls. We will assess pathophysiological relevance by immune cell biology studies.
Structural dynamics associated with intermediate formation in an archetypal conformational disease MP Nyon, L Segu, LD Cabrita, G Lévy, J Kirkpatrick, BD Roussel, AOM Patschull, R Kerr, UI Ekeowa, TE Barrett, N Kalsheker, M Hill, K Thalassinos, DA Lomas, J Christodoulou, B Gooptu Structure 2012, 20:504-12
Conformational pathology of the serpins – themes, variations and therapeutic strategies B Gooptu and DA Lomas Annual Review of Biochemistry 2009, 78:147-176
A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches VJ Patel†, K Thalassinos, Slade, S. E., Connolly, J. B., Crombie, A., Murrell, J. C. and Scrivens, J. H. † joint first authors Journal of Proteome Research (2009) 8:3752-3759