The mechanism by which tissues such as long bones, the jaw, kidney tubules and the inner ear, elongate is by a cell intercalation that is not well understood. The Green lab’s novel, self-elongating cell aggregate system allows size, geometry and cell type composition of the initial cell aggregate can be exquisitely controlled and subsequent cell behaviours observed by confocal timelaspe microscopy. This will be used to test the engineering parameters of elongation compared with those of a computer model developed by the Miodownik group to test our assumptions and improve our understanding of tissue-shaping processes in vivo.
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Ninomiya H, Elinson RP, Winklbauer R.(2004) Antero-posterior tissue polarity links mesoderm convergent extension to axial patterning. Nature 430(6997):364-7
Ninomiya H., Winklbauer R. (2008). Epithelial coating controls mesenchymal shape change through tissue-positioning effects and reduction of surface-minimizing tension. Nat. Cell Biol. 10, 61-69.
Hopyan S, Sharpe J, Yang Y. (2011) Budding behaviors: Growth of the limb as a model of morphogenesis. Developmental Dynamics. 240(5):1054-62.