Cancer is a very devastating disease: more than one in three people in the UK will develop cancer in their lifetime (2011, Cancer Research UK). PI3-kinase is frequently mutated in cancer and regulates key processes including chemotaxis, cell growth, proliferation, and survival. The lipid product of PI3-kinase, Phosphatidylinositol (3,4,5)-trisphosphate (PIP3), controls these pathways by recruiting downstream effectors containing PIP3 binding PH domains. In this project we will characterize a potentially unique, novel, and general mechanism of regulating the recruitment of PH domain-containing effectors to the lipid PIP3 by a combination of confocal live cell imaging, bioinformatics, and biophysical approaches.
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